An impedance method was developed as a rapid, convenient method to enumerate pure culture of Yersinia
enterocolitica. Cultureswere incubated in trypticase soy broth (TSB) at 30 °C. The BacTrac™4000 microorganism
growth analyserwas used to detect impedance change of TSB representing bacteria cell numbers in the samples.
Good correlationswith standard plate countswere obtained (r2 N 0.95). This method is also reliable to enumerate
Y. enterocolitica growing in biofilms attached to stainless steel. Compared with a standard plate count which involves
dislodging biofilms from surfaces, this method is more convenient saving both time and effort. Therefore,
it will be useful to study the conditions required for the biofilm growth and control of Y. enterocolitica that could
be applied to the pork industry.