Five fruit from each tree were washed with distilled water,
homogenized and kept at room temperature (21 ◦C) for 6 h. The
fruit samples were kept in 50 mL tubes at −20 ◦C for biochemical
analysis. A 1 g sample was taken from each fruit sample and 5 mL
methanol was added to each sample. The tests were performed at
6 h intervals.