Molecular weights of the
polypeptides were estimated by using low molecular weight standards (Amercham Biosciences,
Sweden): phosphorylase B (94.0 kDa), bovine albumin (66.0 kDa), ovalbumin (45.0 kDa), carbonic
anhydrase (30.0 kDa), soybean trypsin inhibitor (20.1 kDa), and α-laktalbumin (14.4 kDa).