Animals
Imprinting Control Region (ICR) male mice (age, 6 wk; weight, 28e32 g) were used in experiments evaluating the size and weight of the testis. Thirty male mice were divided into six equal groups (Fig. 1). Each group received saline or red ginseng (300 mg/kg, oral administration); the busulfan control group received saline and busulfan (40 mg/kg, intraperitoneal injection); all the other groups received KRGE orally (100 mg/kg, 200 mg/kg, or 300 mg/kg); in addition, these three groups also received busulfan intraperitone- ally (40 mg/kg) [11]. All groups received KRGE or saline orally for 5 wk. Mice were treated with a single intraperitoneal injection of busulfan 1 wk prior to performing the study. All experiments were conducted in accordance with the National Institutes of Health Guide of Laboratory Animals. The study protocol was approved by the Institutional Animal Care and Use Committee of the Konkuk University (Seoul, Korea).