The effect of biofloc size on the nutritional composition of the flocs and the nitrogen utilization by white shrimp
(Litopenaeus vannamei), red tilapia (Oreochromis niloticus) and mussels (Perna viridis) was investigated. Biofloc
was collected from a shrimp culture unit and labeled with (15NcH4)2SO4. The flocs were sieved grouping them
into 4 different size classes (un-sieved, b48 μm, 48–100 μm, and N100 μm) and subsequently offered to shrimp,
red tilapia and mussels. The biofloc class of N100 μm contained the highest levels of protein (27.8%) and lipid
(7.5%), whereas the biofloc of b48 μm seemed to be richest in essential amino acids. Based on the Essential
Amino Acid Index (EAAI), biofloc produced in this study can be considered as a good quality protein source for
shrimp (0.93–0.97) and a useful protein source for tilapia (0.83–0.90) and mussel (0.81–0.88). The total amount
of nitrogen that could be derived from biofloc was the highest when the biofloc was larger than 100 μm, i.e. 4.06
g N/kg shrimp, 3.79 g N/kg tilapia, and 1.17 g N/kg mussel, respectively. The nitrogen recovery from the biofloc,
however, was the highest when the floc was b48 μm. Overall, this study showed that biofloc consumption by
shrimp, red tilapia and mussels occurs irrespective of floc size but that floc size can play an important role in
the quality of biofloc in terms of nutritional composition and nitrogen retention by the animals.