L. monocytogenes LGB and two Z. bailii strains were used in this
study. The yeasts were recovered from raw fruits and identified by
typical phenotypic and genotypic procedures, while L. monocytogenes
LGB was isolated from cauliflower (Scolari & Vescovo
2004). Frozen cultures of the Listeria strain were subcultured
three times in TSBYE (Tryptone Soya Broth Yeast Extract) at 37 C,
then, stationary phase cells were treated at 400 MPa for 20 min in
fresh medium (eight exposition cycles) to increase their baroresistance.
Survivors from the last exposition cycle were recovered
from TSAYE (Tryptone Soya Agar Yeast Extract) plates, cultured in
TSBYE and utilized in this study.