The cDNA fragments were subcloned to pGEM-T Easy Vector (Promega Corporation, Madison, Wis), and RNA probes were synthesized with the Dig RNA labeling kit (SP6/T7) (Roche Molecular Biochemicals, Tokyo, Japan) and used for hybridization
The cDNA fragments were subcloned topGEM-T Easy Vector (Promega Corporation, Madison,Wis), and RNA probes were synthesized with the DigRNA labeling kit (SP6/T7) (Roche Molecular Biochemicals,Tokyo, Japan) and used for hybridization