Few drops of Molisch’s reagent was added to each of the portion dissolved in
distilled water, this was then followed by addition of 1 ml of conc. H2SO4 by the side of the test tube. The mixture
was then allowed to stand for two minutes and then diluted with 5 ml of distilled water. Formation of a red or dull
violet colour at the interphase of the two layers was a positive test