The aim of this research was to determine the potential of immobilised digestive lipase from Chinook
salmon (Oncorhynchus tshawytscha) to generate flavour compounds in milk. The lipase was immobilised
on hydrophobic resin (Toyopearl Butyl) and used to hydrolyse milk lipids in a batch reactor. The lipase
was stable when immobilised and there was no significant resin fouling or enzyme inhibition between
cycles. Eight cycles were achieved before the hydrolysis rate dropped significantly because of physical
losses of the immobilised lipase. The immobilised lipase showed the highest specificity towards shortchain
fatty acids butanoic and hexanoic acids, the main dairy product flavour and odour compounds.
Based on the performance of the reactor, and the ability of the lipase to alter free fatty acid composition
and sensory characteristics of milk, the immobilised salmon lipase has potential applications in developing
dairy products with unique flavours.