2.2. Ethylene treatment, effect of 1-MCP
Detached flowers were placed in airtight plastic chambers
(37 cm×47 cm×35 cm). An appropriate volume of ethylene was
applied through an injecting port, and brought to a final concentration
of 0.4LL−1. Flowers were exposed to ethylene at 25 ◦C for
24 h. Control flowers were placed in identical chambers, without
ethylene.
If applied, 1-methylcyclopropene (1-MCP) treatment was given
prior to ethylene. 1-MCP was generated by adding water to
EthylBloc® (Smartfresh, Springhouse, PA) powder, placed in a vial in
a chamber similar to the ones used for ethylene treatment. Allowing
water into the vial with Ethylbloc® powder resulted in a final concentration
of 500 nL L−1 of 1-MCP gas in the chamber. Flowerswere
exposed to 1-MCP for 3 h. Control flowers were placed in identical
chambers, without 1-MCP.