The mixture was incubated at 37oC in a shaking water bath (WNB 14 Model; Mem mert,Schwabach, Germ any) for 2 hr. At the end of the incubation period, a dialysis bag containing 20 mL PIPES buffer (0.15 N) was placed into the tube. Following 30 min incubation at 30oC in a shaking water bath, 5 mL of the pancreatin/bile mixture (0.5 g pancreatin + 3 g bile extract/250 mL 1 N NaHCO3) was added and the incubation continued for another 2