using tannic acid as a carbon source [6]. Generally, the
production of this enzyme occurs during the first steps of
microbial growth because tannase is a primary metabolite
[13,20]. According to Aguilar et al. [21], the maximal
tannase production by Aspergillus sp. is attained between
1 to 3 days of cultivation, as observed for the Aspergillus
sp. GM4 extracellular tannase. The decrease in the tannase
production after three days of cultivation may be
due to the reduction in substrate availability and the secretion
of proteases that can act on secreted tannase