Simple linear regression curve for standard capsaicin by HPLC
The simple linear regression curve was plotted using standard capsaicin purchased from Sigma Chemicals. A stock solution of 1mg/ml capsaicin per milliliter of ethanol was prepared and different concentrations of 1000 µg, 600 µg, 200 µg, 50 µg and 1 µg were prepared. Standard curves for capsaicin and dihydrocapsaicin were plotted using concentration versus peak area. Total capsaicinoids in the test samples were quantified using linear equation