The peanut paçoca was macerated in a cold
mill (6°C) until the formation of fine grained flour,
of about 40 mesh. Total proteins of this flour were
extracted in Tris-HCl buffer 50 mM, pH 7.5, at a ratio
of 1:10 (w/v). The mixture was subjected to constant
stirring, for 3 hours at room temperature. Afterwards,
the material was centrifuged at 12,000 g for 30 min at
4oC. The supernatant was named Crude Extract (CE)
and its precipitate was called (PCE).