2.4. Isolation and purification of different microorganisms
Ten grams of the ground bagasse was added under aseptic conditions to 90 mL of sterile saline (8.5 g/L NaCl) in 250 mL conical flasks. The flasks were shaken at 200 rpm for 60 min. After
that, the suspensions were serially diluted to 10 1 and grown
using LB, PD and WH agar plates for bacteria, fungi and yeast,
respectively.