Sperm quality
Sperm motility was determined subjectively using light microscope
(Olympus, Japan) at 400 magnification. Samples were activated
by mixing 1 ll of sperm with 20 ll activation solution (0.3%
NaCl) on a glass slide. The motility percentages were defined as the
percentage of spermatozoa moving in a forward motion every 20%
motile increment (i.e., 0, 20%, 40%, 60%, 80%, and 100%) [34