Thermotolerant yeast was isolated from overripe grapes. The
overripe grape was crushed and 1 g of pulp was suspended in
2 ml sterile saline, thoroughly mixed and allowed to settle. A loopful of supernatant was streaked on enrichment media containing
(g/L): xylose 20.0; malt extract 3.0; yeast extract 3.0; peptone
5.0; (MXYP) agar 20.0 at pH 6.0 ± 0.2 and was incubated at 30,
40 and 50 C for 48 h. Isolated colonies of microorganism growing
at high temperature (40 C) were picked up and subcultured on
slants and these isolates were screened for their biochemical characterization. The isolated yeast was efficient in using xylose glucose, arabinose and mannose as carbon source. The yeast strain
is maintained on MXYP slants