2.7.2. Treatment of Inoculated Seeds with Plant Extracts
The most promising concentrations of plant extracts in in-vitro were used in in-vivo.
Twenty five per cent suspensions of extracts of Azadirachta indica, Aloe vera, Allium sativum, Coffee arabica, Camellia sinensis, Datura stramonium, Nicotiana tabacum and Zingiber officinalis were prepared by soaking 15 g of plant parts (ground leaves and seeds) in 60 ml of SDW.
Ten per cent suspension of Nicotiana tabacum extracts was also prepared by soaking 6 g of leaves in 60 ml of SDW for 20 h [22]. The extracts were filtered using double-layered cheesecloth and the volume was adjusted to 60 ml with SDW and put in 80 ml scotch bottle with cover.
The extracts were then kept at 4˚C until used.