Time dependencies of rapeseed oil conversion to FAEE by
Lipozyme TL HC are shown in Fig. 4 (see also Section 2.2.2). The
schemes of 96% ethanol supply slightly varied, but followed the
general pattern: some amount added at the very beginning, whereupon
the substrate was added continuously over 1.5–4 h (see
legend to Fig. 4B). The reactions were monitored by either TLC
staining (Fig. 4A and B, closed symbols, all reactants) or GC analysis
(Fig. 4B, open symbols, only biodiesel). Both monitoring methods
showed the superimposed reaction profiles for biodiesel, where