Cucumber scientific name is Cucumis sativus L., chromosome number 2n = 14, annuals, climbing vines, The plant is a natural cross pollination by wind and insects but selfing 1-47 %, by nature cucumber monoecious plant . (เฉลิมเกียรติ และภัสรา, 1996) The cucumber can production in all country in Thailand and cultivation for the trade were located in the north and northeast. (นิพนธ์,ม.ป.พ.) Information from Department of Agriculture 2013 in Thailand have planting area of cucumber 26 953 rai and average price of 11.90 baht / kg. At present, cucumber are mostly grown from F1 hybrids, which requires the development of the parents to inbred lines. This study is part of a project to develop inbred purification crop 4 of Green World Genetic (Thailand) Ltd.,. To study on plant characteristics for breeder use of selected varieties according to the needs of domestic and foreign markets, such as India and China. By selection of varieties that need to be more consistent to inbred purification.