2.3. Animals and Housing. All ICR (Imprinting Control Region)
mice and Wistar rats were obtained from the National
Laboratory Animal Center (Mahidol University, Nakorn
Pathom, Thailand). They were housed in the conventional
hygienic animal rooms of the Laboratory Animal Center,
Department of Medical Sciences, Nonthaburi, Thailand.
Room conditions were maintained at 25∘C, 60% humidity
and 12 hour-light-dark cycle. The animals were given a
commercial pellet diet 082 CP feed (Perfect Companion
Group, Thailand) and clean water ad libitum. Prior to the
experiment, the animals were allowed two weeks to acclimate
to the environment.