Isolates
Grape isolates of B. cinerea isolate 1440, A. alternata isolate
22F3, A. niger isolate 36D2 and R. stolonifer were grown on
potato dextrose agar for 2 weeks. Spores were rubbed from
the agar surface with a glass rod after a small volume of
sterile 0Æ05% (v/v) surfactant (Triton X-100) was added.