be effective, there are many disadvantages, including high cost andtime demands, or difficulty in scale-up. Alcohol precipitation can beused as a pretreatment process, and further treatments are neededto obtain APS with high purity. Ion-exchange chromatography cou-pled with gel permeation chromatography for the purification ofAPS will require more cost and time. Membrane separation is aneffective method for the purification of APS, but the membrane iseasily ruined by aloe gel with high viscosity. In our previous stud-ies, an ionic liquid-based aqueous two-phase system (IL-ATPS) wasused for simultaneous extraction and purification of APS and pro-tein [15]. IL-ATPS is an effective method, but unlike TPP extraction,pretreatment via alcohol precipitation was needed to obtain thecrude APS with protein being discarded as an impurity. Further-more, IL was expensive, and protein extracted into the IL-rich phaseshould be separated from IL.