Gas chromatography- mass spectroscopy (GC-MS)
In the structure elucidation of sophorolipids by Candida batistae, for the confirmation of fatty acids, the sample was investigated for GC-MS analysis. The major peak at Retention Time (RT) 51.5 min. was supposed due to be 18-hydroxyoctadecenic acid and this was confirmed by comparing the sample with the authenticated sample produced by Starmerella bombicola. The Fragmentation pattern of