Diagnostic Investigation
Each dog was evaluated with a complete history, including any known exposure to vaccinations, drugs or toxins, physical examination, CBC, slide agglutination test, direct Coombs' test, serum chemistry, urinalysis, coagulation profile (prothrombin time, activated partial thromboplastin time), 3-view thoracic radiography, abdominal ultrasonography, heartworm antigen testing, and polymerase chain reaction testing for vector-borne diseases