.4. Development of the phytic acid

.4. Development of the phytic acid purification technique

An analytical procedure for the purification of PA (Fig. 1) was developed after establishing the conditions according to experimental design (Section 2.3) of extraction for 1 h at 25 °C (shaker model MA 830/A, Marconi, São Paulo, Brazil) with 1.0 N HCl and a concentration of rice bran of 0.1 g mL−1. The pH of the extract containing PA was adjusted to 4.5 with a solution of 4 M NaOH, the rice protein's isoelectric point (Gupta et al., 2008), and the extract was centrifuged at 2000 × g for 10 min (model BR4i, Jouan, Saint Herblain, France). A supernatant (S1) and pellet (P1) were obtained.

ผลลัพธ์ (ไทย) 1:

0.4 Development of phytate acid purification step analysis for compounds of PA (Fig. 1) was developed to determine the conditions of the experimental design (section 2.3) makes for h 1 at 25 ° C (Shakers. Model MA 830 / A, Marconi Sao Paulo, Brazil) with 1.0 N HCl concentration of rice bran mL-1 0.1 G PH Extract the forest was fined 4.5 troubleshooting 4 M NaOH, isoelectric point of the protein (rice. Gupta et al., 2008), and centrifuged at 2000 g extract has bought in 10 minutes (version BR4i, Jouan, St Herblain France) supernatant (S1) and tablets (P1) received.

ผลลัพธ์ (ไทย) 2:

0.4 Development of phytic acid Analysis Techniques for Purification Process for the Purification of PA (Figure 1). It was developed after the creation of conditions to be based on the design of experiments (2.3) extraction for one. H at 25 ° C (a form of Spin MA 830 / A, Marconi, Sao Paulo, Brazil) 1.0 N HCl and concentration of Rice Bran 0.1 G ML-1 pH of the Extract with PA adjusted 4.5. with a Solution of 4 M NaOH at Isoelectric protein Media (Gupta et al., the 2008th) and the extracts were Centrifuged at 2000th × G for 10 min (Model BR4i, Jouan, Saint Herblain, France) Clear. (S1) and tablets (P1) received.

ผลลัพธ์ (ไทย) 3:

4. Development of phytic acid pure technical analysis procedure for the purification of forests (Figure 1), which was developed after the creation of conditions for the design of experiments (Section 2) extraction for one hour at a temperature of 25. ° C (the gearbox to 830 /, Marco Ne, Sao Paulo, Brazil) 1.0 N HCl and concentration of oil, rice bran ml 0.1 G - 1

pH of the mixture of extracts from PA adjusted 4.5 with a solution of 4 M NaOH. Rice protein isolates dielectric points (Gupta et al., 2008), and separate the 2 × G 10 minutes (a br4i jouan, saint, herblain, France) is long (S1) and tablets (P1) at. get