3.1.5. Expression of proteins involved in apoptosis and cell cycle arrest
Western blotting analysis indicated that apoptosis induction in cancer cells by each bran extract was initiated by increase of p53 protein, which was frequently associated with a decrease of inactive caspase-3 (procaspase-3, 35 kDa) protein and decrease of cyclin D1 level (Fig. 6). Among different cancer cell types, HL-60 cell showed higher susceptibility to the treatments than the other cell lines. Cyclin B1 level, however, was reduced only by the DCM extract. Surprisingly, cyclin B1 was increased by the MeOH-extract treatment in every cell type, suggesting the actions of some specific compounds in this extract.