Those samples glutaraldehyde in CaO3for 42 h.
Afterwards samples were placed above 2% OsO4in 20% NaCl for 6 h.
In a graded series of ethanol solutions (50, 70, 90 and 99.5% by volume) both types of samples were dehydrated at room temperature and subsequently embedded in Technovit 7100. Sections of 1.0mm were cut with an RMC Power Tome XL using glass knives; the samples were subsequently placed on glass slides. The sections were stained with Lugol’s iodine solution to visualise the starch phase and light green in acetic acid was used to visualise the protein phase as well as the yeast cells