microbial populations and production of enzymes was limited, thus
contributed to higher substrate limitation and G−/G+ ratio. On the contrary,
higher aliphatic C\H stretching vibration (1406 cm−1
) and
“amide-II” N\H (1550 cm−1
) were detected in alkaline soils, indicating
more easily available substrates and micronutrients, contributed to
higher proportion of gram-negative bacteria.