2. Materials and methods
2.1. Materials
Laccase (EC.1.10.3.2) from ascomycete Myceliophthora thermophila was provided by Novozymes, (Denmark). The 2,2-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) and BSA were obtained from Sigma, Spain. The bleaching reagents and desized cotton were kindly supplied by ACATEL, Portugal.