The influence of far-red light on chrysanthemum growth was studied using a light-emitting diode (LED)-based illuminator. Four groups of chrysanthemum plantlets (Chrysanthemum morifolium Ramat. ‘Ellen’) were simultaneously grown under independently set different illumination regimes. The study showed that far-red light hinders chrysanthemum growth processes in vitro, but increases growth of root length. Meanwhile, with an increase of far-red light photon flux density, the rooting rate of explants decreases. Fresh and dry weight accu- mulation was depended on the rooting processes of chrysanthemum plantlets. The study also shows that phytohormones are involved in dry matter accumulation and rhizogenesis processes. The presence of far-red light reduces the content of all photosynthetic pigments. This shows that by adjusting of the incident spectrum using a multiwavelength solid-state illuminator, the growth process of chrysanthemum culture in vitro can be controlled.