Moreover, reduction of the number of sproutings of the hybrid Citrus paradisix and Poncirus trifoliate popularly known as Citrumelo “Swingle”, was observed, once BAP concentration was increased in the culture medium (Cantagallo et al., 2005). Probably these species stored high concentration of cytokinins. When growth regulators were applied in the culture medium, it can induce negative feedback in the signalling pathways of sprouting induction, besides the phytotoxic effects caused by BAP in high concentrations (Rolli et al., 2011).