presents the comet assay results for human
leukocytes exposed in vitro to different concentrations
of MMS for different exposure times at 37 ◦C. The
mean values for DNA IL, DI, and DF of the treated
human leukocytes were significantly greater from the
controls without MMS, except for the image length
values for the 2 × 10−5M concentration of MMS at
1–6 h, while at the highest MMS concentration (8 ×
10−5 M) there were no viable leukocytes for scoring
at 6 and 24 h. Comet analysis at different exposure
times clearly related increasing concentration to DNA
damage. The damage index of the 24 h control group
was significantly higher than that of the 1, 2 and 6 h
control groups and the damage frequency for the 24 h
control group significantly higher than that of the 2 h
and 6 h control groups and the 1 h control group hada significantly higher damage frequency as compared
to the 6 h control group. There was a general increase
in the DNA damage values with increased exposure
time (24 > 6 > 2 > 1 h) at all MMS concentrations
with the observed values being somewhat higher
than expected, statistically significant differences being
shown in
presents the comet assay results for humanleukocytes exposed in vitro to different concentrationsof MMS for different exposure times at 37 ◦C. Themean values for DNA IL, DI, and DF of the treatedhuman leukocytes were significantly greater from thecontrols without MMS, except for the image lengthvalues for the 2 × 10−5M concentration of MMS at1–6 h, while at the highest MMS concentration (8 ×10−5 M) there were no viable leukocytes for scoringat 6 and 24 h. Comet analysis at different exposuretimes clearly related increasing concentration to DNAdamage. The damage index of the 24 h control groupwas significantly higher than that of the 1, 2 and 6 hcontrol groups and the damage frequency for the 24 hcontrol group significantly higher than that of the 2 hand 6 h control groups and the 1 h control group hada significantly higher damage frequency as comparedto the 6 h control group. There was a general increasein the DNA damage values with increased exposuretime (24 > 6 > 2 > 1 h) at all MMS concentrationswith the observed values being somewhat higherthan expected, statistically significant differences beingshown in
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