PCR reactions
were carried out in 25 µl volume containing
25 ng of total genomic DNA from each
sample, 0.2 µl of a single primer, 100 mM of
each dNTPs, 1X PCR buffer (10 mM TrisHCl
pH 8.3, 50 mM KCl, 2 mM MgCl2) and
1 unit DNA Taq polymerase (Roch,
Mannheim, Germany, 1999)