The decontamination effect of UV-C light at increasing fluence up to 1.2 kJ/m(2) was studied with reference to natural microflora and inoculated pathogens in lamb's lettuce wash water. UV-C light dose of 0.4 kJ/m(2) allowed to inactivate most of the native microflora and to achieve more than 5- Log-reductions in inoculated microorganisms (Salmonella enterica, Listeria monocytogenes and Escherichia coli). In multiple washing cycles up to 5, UV-C light treatment allowed to obtain more than 3-log reductions in native microflora in wash water. Spectral properties indicated that, independently on the number of recycling cycles, a considerable amount of UV-C light can penetrate wash water if its thickness is lower than circa 1 cm. (C) 2014 Elsevier Ltd. All rights reserved.