Identification materials were arranged randomly with three
replications of 10 plants each. Resistant cultivar Zhongnong
No. 13 and susceptible cultivar Jinza No. 4 were used as controls.
All seedings were inoculated, at the stage of fully open
first true leaf, with a single strain of Cladosporium cucumerinum.
Both sides of the leaves of the seedlings were sprayed
uniformly using a hand-pumped sprayer to the point of leaf
runoff. Inoculation concentration was 2 · 106 spores/mL. After
inoculation, plants were kept in the dark at 100% relative
humidity (RH) for 24 h, followed by 7 to 10 d at 60%/85% RH
(day/night) at temperatures of 20 to 23 C.