sperm were recovered
from the excised cauda epididymis and allowed to capacitate for
5 min in M199 media containing 0.5% BSA at 37C. Sperm were
scored as motile if any movement was detected. The total number
of sperm and the number of sperm that were motile were determined
[14]. The percentage of motile spermatozoa in each treatment
group was calculated by the Integrated Semen Analysis
System.