3.2. Effect of H. rhamnoides extract on IMR32 cell line
Cytotoxic effect of H. rhamnoides extract on human neural cell
line-IMR32 was evaluated by incubating it with various concentrations
of H. rhamnoides extract (1–1000 lg/ml) as seen in
Fig. 2 (1000 lg/ml data not shown). The toxicity results revealed
a decrease in percentage of viability at higher concentrations of
extract and the IC50 value was found to be 312.63± 2.6 lg/ml.
The viability of cells was least affected at 100 lg/ml hence, chosen
as the highest safe dose for evaluating efficacy of the extract.