Straight line subtraction (Figure 1B) was found to be the best processing method for the calibration development of oil content in intact Safflower seeds. Accordingly, the calibration parameters were developed by using the information from the best spectral region. Using stepwise elimination of outliers from the calibration graph, the efficiency of the method was gradually improved. 1828 selected data points generated from 47 standard samples were finally used to develop the calibration. Calibration was validated by comparing the oil content values estimated by NMR and FT-NIRS calibration methods in 26 samples. Values showed closed relationship (y = 0.5354x +16.093, r2 =0.853) (Figure 2). Although calibration equation based on ground samples are reported to be more accurate than those based on the intact seed sample possibly due to homogeneity of ground sample and surface and size diversity of intact seeds.