2.7. Preparation of emulsions
Oil-in-water emulsions with okra extracts at concentration
0.625% w/v with dispersed-phase (n-hexadecane) volume fraction
4 ¼ 0.2 and under acidic conditions (pH 3.0) were prepared as
described previously (Alba et al., 2013). Briefly, the aqueous phases
were magnetically stirred with hexadecane for 3 min in order to
produce emulsion pre-mixes with oil volume fraction 4 ¼ 0.2 and
0.5% w/v final extract concentration in the entire emulsion. Processing
conditions previously described (Alba et al., 2013) were
used to fabricate emulsions with reproducible particle size distributions
and investigate them during ageing. Consequently, the pre-mix was immediately homogenized (IKA T18 basic, Ultra-Turrax,
Germany) for 1 min. For the determination of the long-term stability,
all emulsions were stored in an incubation chamber at 25 C.