In the microbial food web, microalgae and zooplankton are the richer source of lipids compared to bacteria (Zhukova and
Kharlamenko, 1999). Out of 20 fatty acids detected in biofloc, palmitic acid, cis-vaccenic acid, linoleic acid and oleic acid were the dominant fatty acids, and omega 3 fatty acids detected in trace level. This is in consonancewith the findings of Crab et al. (2010) who reported that biofloc contain palmitic acid, palmitoleic acid and linoleic acid in the highest and omega 3 fatty acids in trace levels. The lower level of PUFA in biofloc may be due to the dominance of heterotrophic bacteria compared to PUFA rich microalgal community (Meyers and Latscha, 1997).