The fluorescences of the assay mixtures were recorded at every 30 s cycle with
the automated BMG FLUOstar Omega microplate reader system.
The complete run was comprised of 250 cycles. This resulted in a
fluorescence decay curve due to the oxidative degradation of fluorescein
by AAPH. The decay of fluorescence was faster in the blank
than in the antioxidant-containing sample extracts, as the antioxidant
protected fluorescein from the damaging effect of AAPH.
The area between the fluorescence decay curve of the blank and
the sample extract was used to calculate the ORAC values of the
samples. Trolox, at different concentrations (5–20 lM), was used
to obtain a standard curve and to compare ORAC values of various
samples. The data were analysed with the data analysis software,
MARS, linked with Omega reader control software.