antioxidant activity and particularly stronger than
ascorbic acid and BHT which were used as
positive controls (Table 2). The crude 70% EtOH
was slightly better on radical scavenging activity
comparing to the Aq. and CH2Cl2 fractions,
respectively. According to the EtOAc fraction
posed the strongest antioxidant activity,
maceration of the seed coat was conducted in an
attempt to shorten the antioxidant extraction.
Although stepwise maceration based on
polarity of the solvents was more convenience, the
obtaining yield was lower than that from stepwise
partition owning to more chemical constituents
were extracted by 70% EtOH respecting to its
higher polarity. Free radical scavenging activity
and total phenolics content were evaluated in
each sample except mac. n-hexane as most of
active compounds have moderate to high polarity
extracted by EtOAc and EtOH. Despite the
extractive yield of mac. EtOAc was less, the
biological activity was more potent than that of
70% EtOH, Aq. and CH2Cl2 fractions from the
partition. Contrary, mac. 95% EtOH extract was
less potent on free radical scavenging activity.
However, considering on the antioxidant activity,
maceration with EtOAc was more feasible and
practical.
Total phenolics content was determined in an
attempt to relate antioxidant activity with the
active principles. Furthermore, it will be more
convenience and practical in quality control of
Tamarind seed extract for applications. In the
partition method, the EtOAc fraction had the
highest phenolics content followed by 70% EtOH,
Aq. and CH2Cl2 fractions, respectively. Moreover,
the presenting antioxidant activity and phenolics
contents of this modified extraction method were
greater than the previous study1). In addition, the
antioxidant activity was related with the phenolics
content (R2 = 0.6507) as shown in Fig. 2. Thus,
higher phenolics content fraction posses a
stronger antioxidant activity. Furthermore,
development of cosmetics containing EtOAc
fraction from partition which posed the best
antioxidant activity and total phenolics content
was done. Four milky base lotions with white and
smooth opaque appearance were preliminary