Endosperm cells isolated from young fruits were cultured on MS medium supplemented with 5 mM BAP, 1 μM NAA and 15% CM or 1000 mg/l YE. The best result for callus induction (70–72%) . The maximum number of shoots formed on the medium containing 1 μM TDZ, or 5 μM BA and 1 μM NAA.
This clearly proved the efficiency of the protocol and it could be used for developing triploids from other desirable diploid lines in mulberry.