One batch of Cabrales cheese was made following the traditional
manufacturing procedure, which involves the use of raw milk but no
commercial starter culture or mould spores. Samples were taken from
the cheese at days 3, 7, 15, 30 and 60 of ripening. Cubes of 10 g from
the cheese core were cut aseptically and homogenized at 45 °C for
1 min in a Colworth Stomacher 400 (Seward Ltd., London, UK) with
90 ml of a sterile 2% (w/v) sodium citrate solution.