Shake flask cultures were used to identify the time requiredto achieve the peak biomass concentration for inoculation of thebioreactor. A maximum biomass concentration of 7.5 g L−1) wasachieved at 28 h on glucose as the sole carbon source. The maximumspecific growth rate of the culture on glucose was 0.38 h−1. At thispoint, palm oil (1%, v/v) was added and incubation was continuedfor a further 12 h to induce the production of lipase.