We have investigated a propanol-based hand rub (Sterillium®, based on 45% propan-2-ol, 30%
propan-1-ol and 0.2% mecetronium etilsulfate) in three variations: with (A) and without (B) a 1 min hand
wash before the disinfection of 1.5 min with immediate sampling after the disinfection; and (C) without a
hand wash before the disinfection but with sampling 1 min after the disinfection. The efficacy of the three
variations was compared to the reference treatment of EN 12791. All experiments were performed in a
Latin-square design with 20 volunteers. Pre- and post-values (immediate and after 3 hr) were obtained
according to EN 12791.