Fig. 4. Effects of alcohol additional strategy and enzyme content on Lipase GH2-catalyzed biodiesel production from microalgae oil. (A) Methanol addition strategy. A 3:1
methanol/oil molar ratio was established by 1-, 2-, and 3-step addition methods. (B) Ethanol addition strategy. A 5:1 ethanol/oil molar ratio was established by 1-, 2-, 3-, 4-,
and 5-step addition methods. (C) Lipase GH2 content and FAME production. (D) Lipase GH2 content and FAEE production.