n vitro tube formation is a commonly used method for analyzing angiogenesis ( Arnaoutova, George, Kleinman, & Benton, 2009). HUVECs are activated with supplement growth factors contained in the media aligned themselves into a network of capillary-liked tubes after 4–6 h in the absence of treatment conditions (Fig. 6A and B). Co-incubation with each fraction at 50 and 100 μg/mL destroyed the tube networks into small fragments; the effect was most pronounced with the EA-rich fraction 3 (Fig. 6G and L). Single EA and GA treatments also produced such tube fragmentation to a lesser extent (Fig. 6C and F).