Phages in the filtrate were
detected by plating 100 μL of the supernatant, using the standard
method for a double-layer agar plaque assay and the PF4 strain of
V. anguillarum as a host; the plates were incubated overnight at 25 °C
to observe the lysis plaques.
al., 2007), bacterial colonization could also have begun inside the fish.